How to do atr correction using essential ftir

These results show that FTIR-ATR spectroscopy is suitable as a high-throughput method for lignin and energy estimations in large data sets. The calibration models were validated by analyses of independent sets of wood samples yielding R 2 = 0.88 and 0.86 for lignin and energy contents, respectively. The best models with high coefficients of determination (R 2 (calibration) = 0.91 and 0.90 R 2 (cross-validation) = 0.81 and 0.79) and low root mean square errors of cross validation (RMSECV = 0.77% and 62 J g -1) for lignin and energy determination, respectively, were obtained after data pre-processing and automatic wavenumber restriction. For the development of calibration models partial least square regression and cross validation was applied to correlate FTIR spectra determined with an attenuated total reflectance (ATR) unit to measured values of lignin or energy contents. Lignin contents of extractive-free poplar wood samples determined by the acetyl bromide method ranged from 23.4% to 32.1%, and the calorific values measured with a combustion calorimeter varied from 17260 to 19767 J g -1. Since lignin is a major energy-bearing compound, we expected that the energy content of wood would be positively correlated with the lignin content. In this study, we investigated the intra-specific variability of lignin and energy contents in extractive-free wood of hybrid poplar progenies ( Populus trichocarpa Р“вЂ” deltoides) and tested if the range was sufficient for the development of quantitative prediction models based on Fourier transform infrared spectroscopy (FTIR).

Therefore, high-throughput methods are needed for rapid screening of wood in large scale samples, e.g., to evaluate the outcome of tree breeding or genetic engineering. However, different applications such as the production of secondary biofuels or combustion for energy production require different wood properties. There is an increasing demand for renewable resources to replace fossil fuels.

How to do atr correction using essential ftir